Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

IMPORTANT

Process of Recombinant DNA Technology- Isolation of DNA and its Amplification: Overview

This Topic covers sub-topics such as Bioreactors, Downstream Processing, Process of Recombinant DNA Technology, Amplification of Gene of Interest using PCR, Cutting of DNA at Specific Locations and, Obtaining the Foreign Gene Product

Important Questions on Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

EASY
IMPORTANT

In which of the following technique the bacterium Thermus aquatics is used:

MEDIUM
IMPORTANT

Colonies of recombinant bacteria appear white in contrast to non-recombinant bacteria because of 

EASY
IMPORTANT

Name the enzyme used for the joining of DNA strand, whether it is ligase or endonuclease?

MEDIUM
IMPORTANT

Write a short note on the role of restriction endonuclease in rDNA technology.

MEDIUM
IMPORTANT

The restriction endonuclease is used to cut the DNA molecules at a specific site.

MEDIUM
IMPORTANT

Which one among the following primer pairs would amplify the fragment of DNA given below?

5'-CTAGTCGTCGAT-N300-GACTGAGCTGAGCTG-3'

3'·GATCAGCAGCTA-(N)300-CTGACTCGACTCGAC-5'

MEDIUM
IMPORTANT

The cell or organism from which the required gene for rDNA technique is taken is called recipient.

EASY
IMPORTANT

The source of Taq polymerase used in PCR is a halophilic bacterium.

EASY
IMPORTANT

The recombinant DNA technology is also popularly called as Genetic engineering or Medical engineering?