Process of Recombinant DNA Technology- Isolation of DNA and its Amplification
Process of Recombinant DNA Technology- Isolation of DNA and its Amplification: Overview
This Topic covers sub-topics such as Bioreactors, Downstream Processing, Process of Recombinant DNA Technology, Amplification of Gene of Interest using PCR, Cutting of DNA at Specific Locations and, Obtaining the Foreign Gene Product
Important Questions on Process of Recombinant DNA Technology- Isolation of DNA and its Amplification
The source organisms that possess Taq polymerase are

In which of the following technique the bacterium Thermus aquatics is used:

Colonies of recombinant bacteria appear white in contrast to non-recombinant bacteria because of

Name the enzyme used for the joining of DNA strand, whether it is ligase or endonuclease?

Write a short note on the role of restriction endonuclease in rDNA technology.

The restriction endonuclease is used to cut the DNA molecules at a specific site.

_____ of the DNA strands is the second step in PCR process.

The full form of PCR is

Which one among the following primer pairs would amplify the fragment of given below?

The cell or organism from which the required gene for rDNA technique is taken is called recipient.

Choose the correct sequence of steps in

Identify the chief enzyme that is involved in technique.

Which among the following does not help in early diagnosis?

The source of Taq polymerase used in PCR is a halophilic bacterium.

Microorganisms can be grown in the bioreactors by

The recombinant DNA technology is also popularly called as Genetic engineering or Medical engineering?

Describe gene amplification (PCR).

PCR technique was developed by ____.

Define PCR.

Explain Annealing.
